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Product Info Summary
| SKU: | LS010849 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of CD3e molecule, epsilon associated protein (CD3EAP) |
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Product info
Product Name
PAF49 (CD3EAP) (NM_012099) Human Over-expression Lysate
SKU/Catalog Number
LS010849
Size
100 µg
Description
Transient overexpression lysate of CD3e molecule, epsilon associated protein (CD3EAP)
Storage & Handling
Cite This Product
PAF49 (CD3EAP) (NM_012099) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010849)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PAF49 (CD3EAP) Human Over-expression Lysates NM_012099.
Western blot validation of overexpression lysate (LS010849) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CD3EAP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CD3EAP (Source: Uniprot.org, NCBI)
Gene Name
CD3EAP
Full Name
DNA-directed RNA polymerase I subunit RPA34
Weight
54.986kDa
Superfamily
eukaryotic RPA34 RNA polymerase subunit family
Alternative Names
A34.5; antisense to ERCC 1; Antisense to ERCC-1 protein; ASE-1ASE1; CASTMGC118851; CD3 epsilon associated protein; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3-epsilon-associated protein; DNA-directed RNA polymerase I subunit RPA34; PAF49RNA polymerase I-associated factor PAF49 POLR1G ASE-1, ASE1, CAST, CD3EAP, PAF49, RPA34 RNA polymerase I subunit G DNA-directed RNA polymerase I subunit RPA34|CD3e antigen, epsilon polypeptide associated protein|CD3e molecule associated protein|CD3e molecule, epsilon associated protein|DNA-directed RNA polymerase I subunit G|RNA polymerase I-associated factor PAF49|antisense to ERCC-1 protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CD3EAP, check out the CD3EAP Infographic
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In this infographic, you will see the following information for CD3EAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PAF49 (CD3EAP) (NM_012099) Human Over-expression Lysate (LS010849)
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No publications found for LS010849
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